HPLC UV Method for Analysis of Cyproheptadine Hydrochloride on Primesep 100 Column

HPLC Method for Cyproheptadine on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Cyproheptadine on Primesep 100 Column by SIELC Technologies

Cyproheptadine is a first-generation antihistamine with the formula C21H21N. It is used primarily in treatment of allergies, insomnia, and serotonin syndrome. It is occasionally used as an appetite stimulant. It works sas the inverse agonist of the histamine H1 receptor. You can find detailed UV spectra of Cyproheptadine and information about its various lambda maxima by visiting the following link.

Cyproheptadine can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and H2SO4 as a buffer. Detection is carried out using UV

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 70/30%
BufferH2SO4  – 0.1%
Flow Rate1 ml/min
DetectionUV 200 nm
LOD*5 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsMedication
Analyzing CompoundsCyproheptadine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cyproheptadine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.