HPLC Method for Analysis of Isoprene on Newcrom R1 Column

HPLC Method for Isoprene on Newcrom R1 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoprene

Isoprene is a volatile organic compound with the molecular formula C5H8. It is most often found in plants and is released as protection from heat stress. It also helps with maintaining cell membrane stability. Isoprene emissions are also the reasons why Blue Ridge Mountains have a blue haze.

Isoprene can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV 222nm.

You can find detailed UV spectra of Isoprene and information about its various lambda maxima by visiting the following link.

ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 60%
BufferNone
Flow Rate1.0 ml/min
DetectionUV 222
LOD*35 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsTerpenoid
Analyzing CompoundsIsoprene

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Isoprene
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.