| CAS Number | 78-79-5 |
|---|---|
| Molecular Formula | C5H8 |
| Molecular Weight | 68.120 |
| InChI Key | RRHGJUQNOFWUDK-UHFFFAOYSA-N |
| LogP | 2.42 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Isoprene
October 3, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Isoprene on Newcrom R1 Column
October 3, 2025
HPLC Method for Isoprene on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoprene
Isoprene is a volatile organic compound with the molecular formula C5H8. It is most often found in plants and is released as protection from heat stress. It also helps with maintaining cell membrane stability. Isoprene emissions are also the reasons why Blue Ridge Mountains have a blue haze.
Isoprene can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV 222nm.
You can find detailed UV spectra of Isoprene and information about its various lambda maxima by visiting the following link.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 60% |
| Buffer | None |
| Flow Rate | 1.0 ml/min |
| Detection | UV 222 |
| LOD* | 35 ppb |
| Class of Compounds | Terpenoid |
| Analyzing Compounds | Isoprene |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Isoprene on Newcrom R1 HPLC column
February 16, 2018
Isoprene can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
