HPLC ELSD Method for Analysis of Cholesterol on Lipak Column

HPLC Method for Cholesterol on Lipak by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Cholesterol

Cholesterol is a compound that is essential for cell function with the molecular formula C27H46O. High levels of cholesterol can lead to heart disease and stroke due to potential accumulation in the arteries. It is caused through a variety of causes includinggenetics, an unhealthy diet, lack of exercise, smoking, and certain medical conditions.

Cholesterol can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and methanol (MeOH) with a formic acid buffer. Detection is performed using ELSD.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

ColumnLipak, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeOH – 99.5%
BufferFormic Acid – 0.5%
Flow Rate0.5 ml/min
DetectionELSD

Class of CompoundsCholestanoid
Analyzing CompoundsCholesterol

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cholesterol
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.