HPLC Determination of Succinic Anhydride and Succinic Acid on SHARC 1 Column

HPLC Method for Succinic anhydride, Succinic Acid on SHARC 1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinic Anhydride and Succinic Acid

Succinic Acid is a dicarboxylic acid with the chemical formula C4H6O4. It is a precursor of some polyesters as well as a component of some alkyd resins. In food, it is typically used as an acidity regulator and flavoring agent. Sulfonic acids like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. You can find detailed UV spectra of Succinic Acid and information about its various lambda maxima by visiting the following link.

Succinic anhydride is a colorless, crystalline compound with the chemical formula C4H4O3. It is used often in the production of polymer resins, especially of the alkyd variety. It has the ability to increase the solubility of various proteins and has other benefits for the pharmaceutical, flavor, and fragrance industries.You can find detailed UV spectra of Succinic anhydride and information about its various lambda maxima by visiting the following link.

Succinic anhydride, Succinic Acid can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.

Condition
Column SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN- 100%*
Buffer No
Flow Rate 1.0 ml/min
Detection UV 210 nm

*Before running, wash the column using 0.5 % phosphoric acid in water and run this MP for a couple of hours. Then switch to pure MeCN.

Description
Class of Compounds Anhydride, Acid
Analyzing Compounds Succinic anhydride, Succinic Acid

 

Application Column

SHARC 1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Succinic Acid
Succinic anhydride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.