| CAS Number | 108-30-5 |
|---|---|
| Molecular Formula | C4H4O3 |
| Molecular Weight | 100.073 |
| InChI Key | RINCXYDBBGOEEQ-UHFFFAOYSA-N |
| LogP | -0.602 |
| Synonyms |
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Applications:
HPLC Determination of Succinic Anhydride and Succinic Acid on SHARC 1 Column
February 8, 2022
Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinic Anhydride and Succinic Acid
Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Succinic anhydride, its acid anhyrdie, is a colorless, crystalline compound used often in the production of polymer resins, often of the alkyd variety. It also has the ability to increase the solubility of various proteins and has other benefits for the pharmaceutical, flavor, and fragrance industries.
Succinic Anhydride and Succinic Acid can be detected in the low UV regime. Using a SHARC 1 hydrogen-bond column and a mobile phase consisting of only acetonitrile (and without a buffer), these two compounds can be separated, detected, and analyzed. This analysis method can be UV detected at 210 nm.
| Column | SHARC 1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN- 100%* |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
*Before running, wash the column using 0.5 % phosphoric acid in water and run this MP for a couple of hours. Then switch to pure MeCN.
| Class of Compounds | Anhydride, Acid |
| Analyzing Compounds | Succinic Anhydride, Succinic Acid |
Application Column
SHARC 1
The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.
Select optionsSuccinic anhydride
HPLC Method For Analysis Of Succinic Anhydride and Succinic Acid on Primesep S2 Column
February 1, 2022
Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinic Anhydride and Succinic Acid
Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Succinic anhydride, its acid anhydride, is a colorless, crystalline compound used often in the production of polymer resins, often of the alkyd variety. It also has the ability to increase the solubility of various proteins and has other benefits for the pharmaceutical, flavor, and fragrance industries.
These two related compounds can be detected in the low UV regime. Using a Primsep S2 HILIC column, which uses hydrogen-bonding as a separation mechanism, and a mobile phase consisting of water and an acetonitrile (ACN) gradient with no buffer, both of these compounds can be separated and retained. This analysis method can be UV detected at 225 nm.
| Column | Primesep S2, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN- 100% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 225 nm |
| Class of Compounds | Anhydride, Acid |
| Analyzing Compounds | Succinic Anhydride, Succinic Acid |
Application Column
Primesep S2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSuccinic anhydride
Separation of Succinic anhydride on Newcrom R1 HPLC column
February 19, 2018
Succinic anhydride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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