Separation type: Liquid Chromatography HILIC
High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinic Anhydride and Succinic Acid
Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Succinic anhydride, its acid anhydride, is a colorless, crystalline compound used often in the production of polymer resins, often of the alkyd variety. It also has the ability to increase the solubility of various proteins and has other benefits for the pharmaceutical, flavor, and fragrance industries.
These two related compounds can be detected in the low UV regime. Using a Primsep S2 HILIC column, which uses hydrogen-bonding as a separation mechanism, and a mobile phase consisting of water and an acetonitrile (ACN) gradient with no buffer, both of these compounds can be separated and retained. This analysis method can be UV detected at 225 nm.
| Column | Primesep S2, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN- 100% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 225 nm |
| Class of Compounds | Anhydride, Acid |
| Analyzing Compounds | Succinic Anhydride, Succinic Acid |
Application Column
Primesep S2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSuccinic anhydride
