HPLC Determination of Oseltamivir on Primesep 100 Column

HPLC Method for Oseltamivir on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Oseltamivir.

Oseltamivir belongs to a class of drugs called antivirals, which are used to treat infections caused by viruses. It is used in the treatment of the infection caused by the flu virus (influenza A and influenza B) and may also be used to prevent and treat swine influenza A.  It is recommended that it be used within the first 48 hours of the infection’s symptoms. It has the chemical formula C16H28N2O4.

Oseltamivir can be retained on a Primesep 100 column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulfuric acid (H2SO4) as a buffer. UV Detection 200nm.

Condition
Column Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4 – 0.5%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Oseltamivir

 

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Oseltamivir
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.