HPLC Method for Mycophenolate mofetil on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolate mofetil.
Mycophenolate mofetil alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 250nm.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-20%, 10 min |
| Buffer | H3PO4 – 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Hydrophobic, Ionizable, Carbocyclic, Benzoate |
| Analyzing Compounds | Mycophenolate mofetil |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Mycophenolate mofetilApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
