| CAS Number | 128794-94-5 |
|---|---|
| Molecular Formula | C23H31NO7 |
| Molecular Weight | 433.501 |
| InChI Key | RTGDFNSFWBGLEC-SYZQJQIISA-N |
| LogP | 3.2 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Mycophenolate Mofetil
March 12, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Mycophenolate mofetil check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column
May 2, 2019
HPLC Method for Mycophenolic acid, Mycophenolate mofetil on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid, Mycophenolate mofetil.
Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.
| Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4- 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic acid, Mycophenolate mofetil |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Mycophenolic acid
HPLC Determination of Mycophenolate Mofetil on Primesep SB Column
May 1, 2019
HPLC Method for Mycophenolate mofetil on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolate mofetil.
Mycophenolate mofetil alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 250nm.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-20%, 10 min |
| Buffer | H3PO4 – 0.2 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Hydrophobic, Ionizable, Carbocyclic, Benzoate |
| Analyzing Compounds | Mycophenolate mofetil |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
