Mycophenolic acid

Mycophenolic acid structural formula

CAS Number24280-93-1
Molecular FormulaC17H20O6
Molecular Weight320.342
InChI KeyHPNSFSBZBAHARI-RUDMXATFSA-N
LogP3.40
Synonyms
  • Mycophenolic acid
  • (4E)-6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-1,3-dihydro-2-benzofuran-5-yl)-4-methylhex-4-enoic acid
  • 4-Hexenoic acid, 6-(1,3-dihydro-4-hydroxy-6-methoxy-7-methyl-3-oxo-5-isobenzofuranyl)-4-methyl-, (4E)-
  • 24280-93-1
  • 4-Hexenoic acid, 6-(1,3-dihydro-4-hydroxy-6-methoxy-7-methyl-3-oxo-5-isobenzofuranyl)-4-methyl-, (4E)-
  • 4-Hexenoic acid, 6-(1,3-dihydro-4-hydroxy-6-methoxy-7-methyl-3-oxo-5-isobenzofuranyl)-4-methyl-, (E)-
  • 4-Hexenoic acid, 6-(4-hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-, (E)-
  • acide mycophenolique
  • acido micofenolico
  • Lilly 68618
  • Mycophenolinsaure
  • NSC 129185
  • EINECS 246-119-3
  • 6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-4-hexenoic acid (E)-
  • Lilly-68618
  • 4-Methyl-5-methoxy-7-hydroxy-6-(5-carboxy-3-methylpent-2-en-1-yl)-phthalide (E)-
  • NSC-129185
  • Acidum mycophenolicum
  • UNII-HU9DX48N0T
  • (E)-6-(4-Hydroxy-6-methoxy-7-methyl-3-oxo-5-phthalanyl)-4-methyl-4-hexenoic acid
  • Micofenolico acido
  • Mycophenolate
  • Mycophenolsaeure

Applications:

HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column

May 2, 2019

HPLC Method for Mycophenolic acid, Mycophenolate mofetil on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid, Mycophenolate mofetil.

Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer H3PO4- 0.2%
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolic acid, Mycophenolate mofetil

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Mycophenolate mofetil
Mycophenolic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Mycophenolic Acidon Primesep SB Column

May 1, 2019

HPLC Method for Mycophenolic acid on Primesep SB by SIELC Technologies

HPLC Determination of Mycophenolic Acid

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid.

Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.

 

Condition
Column Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 40/60%
Buffer H3PO4- 0.4%
Flow Rate 1.0 ml/min
Detection UV, 226 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolic acid

 

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Mycophenolic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Mycophenolic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Mycophenolic acid on Newcrom C18 HPLC column

Mycophenolic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Mycophenolic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.