HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column

Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.


Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H3PO4- 0.2%
Flow Rate 1.0 ml/min
Detection UV, 220 nm



Class of Compounds
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolate mofetil, Mycophenolic Acid


Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Mycophenolate mofetil
Mycophenolic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.