HPLC Method for Mycophenolic acid, Mycophenolate mofetil on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid, Mycophenolate mofetil.
Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.
Column | Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | H3PO4- 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV, 220 nm |
Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
Analyzing Compounds | Mycophenolic acid, Mycophenolate mofetil |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Mycophenolic acid