HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column

HPLC Method for Mycophenolic acid, Mycophenolate mofetil on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid, Mycophenolate mofetil.

Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.

Condition

Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer H3PO4- 0.2%
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant
Analyzing Compounds Mycophenolic acid, Mycophenolate mofetil

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mycophenolate mofetil
Mycophenolic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.