Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.
| Column | Primesep SB, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H3PO4- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 226 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic Acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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