HPLC Method for Mycophenolic acid on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid.
Mycophenolic acid alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer with UV detection at 226nm.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H3PO4- 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 226 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic acid |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Mycophenolic acidApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
