HPLC Determination of Mycophenolate Mofetil on Primesep SB Column

HPLC Determination of Mycophenolate mofetil
Mycophenolate mofetil alongside other medicines is used as an immunosuppressant to prevent organ rejection after a transplant. THe compound can be retained in HPLC using Primesep SB mixed-mode column that has both hydrophobic and anion-exchange properties. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 250nm.

Condition

Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-20%, 10 min
Buffer H3PO4 – 0.2 %
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Hydrophobic, Ionizable, Carbocyclic, Benzoate
Analyzing Compounds Mycophenolate Mofetil

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Mycophenolate mofetil
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.