HPLC Method for Analysis of Benzalkonium Chloride on Primesep SB Column

HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies

HPLC Method for Analysis of Benzalkonium Chloride on Primesep SB Column
Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C6H5CH2N(CH3)2RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride can be retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using a gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier.  This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 30-70%, 10 min
BufferH2SO4 – 0.2%
Flow Rate0.5 ml/min
DetectionUV 210 nm
Peak Retention Time5.32, 6.93

Description

Class of CompoundsDrug
Analyzing CompoundsBenzalkonium chloride

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzalkonium chloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.