HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant often used as a pharmaceutical preservative and as an antiseptic. Benzalkonium Chloride can be retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using a gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) ionic modifier. This analysis method can be UV detected at 210 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride
Condition
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 30-70%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 5.32, 6.93 |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Benzalkonium chloride |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Benzalkonium chloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
