Fisetin

CAS Number528-48-3
Molecular FormulaC15H10O6
Molecular Weight286.239
InChI KeyXHEFDIBZLJXQHF-UHFFFAOYSA-N
LogP2
Synonyms
  • Fisetin
  • 528-48-3
  • 2-(3,4-Dihydroxyphenyl)-3,7-dihydroxy-4H-chromen-4-one
  • 5-Desoxyquercetin
  • Cotinin
  • Fustel
  • Viset
  • 3,3',4',7-Tetrahydroxyflavone
  • Fisetholz
  • Superfustel
  • Fietin
  • Fustet
  • Junger fustik
  • Ventin sumach
  • Young fustic
  • Zante fustic
  • Superfustel K
  • 3,7,3',4'-Tetrahydroxyflavone
  • Ungarisches gelbholz
  • C.I. Natural Brown 1
  • Young fustic crystals
  • Bois bleu de Honqrie
  • BOIS bleude honqrie
  • C.I. 75620
  • NSC 407010
  • NSC 656275
  • 5-Deoxyquercetin
  • 2-(3,4-Dihydroxyphenyl)-3,7-dihydroxy-4H-1-benzopyran-4-one
  • Natural Brown 1

Applications:

HPLC Method for Analysis of Fisetin

October 10, 2018

HPLC Method for Fisetin on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fisetin 

Fisetin, is a plant polyphenol from the flavonoid group. It can be found in many plants, where it serves as a coloring agent. It is also found in many fruits and vegetables, such as strawberries, apples, persimmons, onions and cucumbers. Fisetin is a natural flavonoid with various benefits, such as antioxidant, anticancer, neuroprotection effects.

Fisetin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid base. Detection is performed using UV.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 25/75%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm

Description
Class of Compounds
  Hydrophobic, Ionizable, Coloring agent,   Polyphenol
Analyzing Compounds Fisetin

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Fisetin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.