HPLC Method for Analysis of Fisetin

Fisetin, is a plant polyphenol from the flavonoid group. It can be found in many plants, where it serves as a coloring agent. It is also found in many fruits and vegetables, such as strawberries, apples, persimmons, onions and cucumbers. Fisetin is a natural flavonoid with various benefits, such as antioxidant, anticancer, neuroprotection effects.  


Column Newcrom R1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 25/75%
Buffer H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Hydrophobic, Ionizable, Coloring agent,   Polyphenol
Analyzing Compounds Fisetin
Application Analytes: