HPLC Method for Analysis of Fisetin

Fisetin, is a plant polyphenol from the flavonoid group. It can be found in many plants, where it serves as a coloring agent. It is also found in many fruits and vegetables, such as strawberries, apples, persimmons, onions and cucumbers.
Fisetin is a natural flavonoid with various benefits, such as antioxidant, anticancer, neuroprotection effects.

 

Condition

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
 Hydrophobic, Ionizable, Coloring agent,   Polyphenol
Analyzing Compounds Fisetin

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Fisetin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.