| Molecular Formula | C23H36N2O2 |
|---|---|
| Molecular Weight | 372.5 |
| InChI Key | DBEPLOCGEIEOCV-WSBQPABSSA-N |
| LogP | 2 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Finasteride
July 3, 2024
Access the UV-Vis Spectrum SIELC Library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
HPLC Method for Separation of Finasteride, Minoxidil and Potassium sorbate on Primesep 100 Column
February 3, 2023
HPLC Method for Separation of Finasteride, Minoxidil and Potassium sorbate on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Finasteride, also known as Proscar or Propecia, is a popular oral medication used to prevent hair loss and treat enlarged prostates (also known as benign prostatic hyperplasia, BPH). Minoxidil is another drug used to treat male pattern hair loss, as well as high blood pressure, and can be taken both orally or topically. Potassium Sorbate is a popular preservative used in a wide variety of foods, wines, and personal-care products. These 3 compounds can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid (H2SO4). This analytical method can be UV detected at 225 nm with high resolution and peak symmetry. The retention times and elution order of these compounds can be modified by altering the relative concentrations of MeCN and H2SO4.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Finasteride, Minoxidil and Potassium sorbate
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 225 nm |
| Peak Retention Time |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Finasteride, Minoxidil, Potassium sorbate |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Minoxidil
Potassium sorbate
