Access the UV-Vis Spectrum SIELC Library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

HPLC Method for Separation of Finasteride, Minoxidil and Potassium sorbate on Primesep 100 by SIELC Technologies

Finasteride, also known as Proscar or Propecia, is a popular oral medication with the chemical formula C₂₃H₃₆N₂O₂. It is primarily used to prevent hair loss and treat enlarged prostates (also known as benign prostatic hyperplasia, BPH). On occasion, it is used to treat excessive hair growth. It works as a 5a-reductase inhibitor, which leads to reduced formation of dihydrotestosterone (DHT) from testosterone. You can find detailed UV spectra of Finasteride and information about its various lambda maxima by visiting the following link.

Minoxidil is a drug with the chemical formula C₉H₁₅N₅O. It is used to treat male pattern hair loss, as well as high blood pressure, and can be taken both orally or topically. It is an adenosine 5′-triphosphate (ATP)-sensitive potassium channel opener, which leads to hyperpolarization of cell membranes.. That allows for more oxygen, blood, and nutrients to follicles. You can find detailed UV spectra of Minoxidil and information about its various lambda maxima by visiting the following link.

Potassium Sorbate is a popular preservative with the chemical formula C₆H₇KO₂. It is used in a wide variety of foods, wines, and personal-care products. It works through inhibiting molds, yeasts, and various other microbes. You can find detailed UV spectra of Potassium Sorbate and information about its various lambda maxima by visiting the following link.

Minoxidil, Potassium sorbate, Finasteride can be retained, separated, and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid (H2SO4). This analytical method can be UV detected at 225 nm with high resolution and peak symmetry. The retention times and elution order of these compounds can be modified by altering the relative concentrations of MeCN and H2SO4.

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