| CAS Number | 76824-35-6 |
|---|---|
| Molecular Formula | C8H15N7O2S3 |
| Molecular Weight | 337.441 |
| InChI Key | XUFQPHANEAPEMJ-UHFFFAOYSA-N |
| LogP | -0.640 |
| Synonyms |
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Applications:
HPLC Determination of Famotidine in Tablets on Primesep 100 Column
November 27, 2018
HPLC Method for Famotidine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Famotidine
Famotidine is an over-the-counter histamine H2-receptor antagonist which is often used to decrease the amount of acid in the stomach. It works through blocking histamine-2 receptors in the stomach lining.
Famotidine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 266 nm |
| Class of Compounds |
Drug, Antacid, Antihistamine, Zwitterionic, Hydrophobic, Ionizable |
| Analyzing Compounds | Famotidine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Famotidine on Newcrom R1 HPLC column
February 16, 2018
Famotidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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