HPLC Method for Famotidine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Famotidine
Famotidine is an over-the-counter histamine H2-receptor antagonist which is often used to decrease the amount of acid in the stomach. It works through blocking histamine-2 receptors in the stomach lining.
Famotidine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 266 nm |
| Class of Compounds |
Drug, Antacid, Antihistamine, Zwitterionic, Hydrophobic, Ionizable |
| Analyzing Compounds | Famotidine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended




