| CAS Number | 125-29-1 |
|---|---|
| Molecular Formula | C18H21NO3 |
| Molecular Weight | 299.370 |
| InChI Key | LLPOLZWFYMWNKH-CMKMFDCUSA-N |
| LogP | 1.42 |
| Synonyms |
|
Applications:
HPLC Method for Analysis of Vicodin (Acetaminophen & Hydrocodone)
May 10, 2016
| Column | Primesep 200, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 5-50% |
| Buffer | Gradient H3PO4 – 0.05-0.3%, 5 min, 4 min hold |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Analgetic, Acid, Hydrophilic, Ionizable, |
| Analyzing Compounds | Acetaminophen, Hydrocodone |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHydrocodone
Vicodin
HPLC Separation of Codeine, Oxycodone, and Hydrocodone on Primesep 100
November 21, 2010
Codeine, oxycodone, and hydrocodone are analgesics with hydrophobic and basic properties. All three compounds are used as strong pain killers and cough suppressant. These compounds are controlled substances according to DEA. We developed a simple method for separation of these narcotics on Primesep 100 and Primesep 200 mixed-mode columns. All three compounds are separated by reversed-phase and cation-exchange mechanism, based on the difference in hydrophobic and ionic properties. This HPLC approach can be used to develop and validate a generic method for analysis of analgesics and other drugs. Compounds are monitored by common detection techniques.
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Hydrocodone
Oxycodone
HPLC Separation of Codeine, Oxycodone, and Hydrocodone on Primesep 200
November 21, 2010
Codeine, oxycodone, and hydrocodone are analgesics with hydrophobic and basic properties. All three compounds are used as strong pain killers and cough suppressant. These compounds are controlled substances according to DEA. We developed a simple method for separation of these narcotics on Primesep 100 and Primesep 200 mixed-mode columns. All three compounds are separated by reversed-phase and cation-exchange mechanism, based on the difference in hydrophobic and ionic properties. This HPLC approach can be used to develop and validate a generic method for analysis of analgesics and other drugs. Compounds are monitored by common detection techniques.
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Hydrocodone
Oxycodone
