2-Amino-5-Methylthiadiazole

2-Amino-5-Methylthiadiazole structural formula

CAS Number108-33-8
Molecular FormulaC3H5N3S
Molecular Weight115.151
InChI KeyHMPUHXCGUHDVBI-UHFFFAOYSA-N
LogP0.284
Synonyms
  • 1,3,4-Thiadiazole, 2-amino-5-methyl-
  • 5-Methyl-1,3,4-thiadiazol-2-amine
  • 1,3,4-Thiadiazol-2-amine, 5-methyl-
  • 108-33-8
  • 2-Amino-5-methylthiadiazole
  • 2-Amino-5-methyl-1,3,4-thiadiazole
  • EINECS 203-573-7
  • 2-Methyl-5-amino-1,3,4-thiadiazole
  • 5-Methyl-1,3,4-thiadiazol-2-amine
  • 5-Methyl-1,3,4-thiadiazole-2-amine
  • NSC 137228
  • 1,3,4-Thiadiazol-2-amine, 5-methyl-

Applications:

Separation of Model Compounds in Reversed-Phase and Mixed-Mode

April 25, 2019

HPLC Method for Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole.

Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.

Condition
Column Solid-Core Primesep 100, 3.2 x 50 mm, 2.7 µm, 90 A, dual ended
Mobile Phase Gradient  MeCN – 10-60%, 5 min
Buffer Gradient AmFm pH 3.5- 30 – 70 mM, 5 min
Flow Rate 1.2 ml/min
Detection UV, 270 nm

 

Description
Class of Compounds
 Drug,  Basic, Hydrophilic, Hydrophobic, Ionizable.
Analyzing Compounds Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole

 

Application Column

Solid-Core Primesep 100

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 2.7 µm
Pore Size: 90 A
Attribute: none
Column options: dual ended

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Application Analytes:
2-Amino-5-Methylthiadiazole
2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.