Access the UV-Vis Spectrum SIELC Library

UV-Vis Spectrum of Carnosine (beta-alanyl-L-histidine). Absorption starts high at 200 nm, plateaus around 210 nm, and quickly falls off and bottoms out around 240 nm.

If you are looking for optimized HPLC method to analyze Carnosine (beta-alanyl-L-histidine) check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 by SIELC Technologies

Carnosine is a dipeptide molecule with the chemical formula C₉H₁₄N₄O₃. It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.

Carnosine (beta-alanyl-L-histidine) can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.

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HPLC Method for Carnosine (beta-alanyl-L-histidine) on BIST B+ by SIELC Technologies

Carnosine is a dipeptide molecule with the chemical formula C₉H₁₄N₄O₃. It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.

Using SIELC’s newly introduced BIST™ method, Carnosine (beta-alanyl-L-histidine) can be retained on a positively charged, antion-exchange BIST B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged peptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Carnosine can be separated, retained, and UV detected at 205 nm.

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