HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column

HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column
HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column by SIELC Technologies

Carnosine is a dipeptide molecule with the chemical formula C9H14N4O3. It is synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer. It is found in muscles and brain tissue, but is produced in the liver. Some use it as a nutritional supplement, but it can be used in topical applications to reduce wrinkles.

Carnosine (beta-alanyl-L-histidine) can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferTFA – 0.6%
Flow Rate1.0 ml/min
DetectionUV 215 nm
Peak Retention Time5.21 min

Description

Class of CompoundsPhenols
Analyzing CompoundsCarnosine (beta-alanyl-L-histidine)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carnosine (beta-alanyl-L-histidine)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.