HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column

HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column
HPLC Method for Analysis of Carnosine (beta-alanyl-L-histidine) on Primesep 100 Column by SIELC Technologies

Carnosine is a dipeptide molecule synthesized from beta-alanine and histidine. It is a naturally occurring pH buffer with antioxidant properties found in muscles. This dipeptide can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 215 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Carnosine (beta-alanyl-L-histidine)

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferTFA – 0.6%
Flow Rate1.0 ml/min
DetectionUV 215 nm
Peak Retention Time5.21 min

Description

Class of CompoundsPhenols
Analyzing CompoundsCarnosine (beta-alanyl-L-histidine)

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Carnosine (beta-alanyl-L-histidine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.