| CAS Number | 28772-56-7 |
|---|---|
| Molecular Formula | C30H23BrO4 |
| Molecular Weight | 527.414 |
| InChI Key | OWNRRUFOJXFKCU-UHFFFAOYSA-N |
| LogP | 6.1 |
| Synonyms |
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Applications:
HPLC Chiral Separation of Bromadiolone on Cyralis CM Column
June 18, 2026
HPLC Method for Analysis of Bromadiolone on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromadiolone.
Bromadiolone is a an anticoagulant with the chemical formula C30H23BrO4. It works as a rodenticide which has a tendency to accumulate in the liver of the poisoned organism. It is a second-generation 4-hydroxycoumarin derivative. Vitamin K1 is considered to be an antidote to the pesticide. You can find detailed UV spectra of Bromadiolone and information about its various lambda maxima by visiting the following link.
This application describes a normal-phase chiral HPLC method for the separation of Bromadiolone enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.
Bromadiolone can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Hexane/IPA/TFA – 87/13/0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Resolution | (1-2): 9.90, (2-3): 1.69, (3-4): 2.20 |
Description
| Class of Compounds | Pesticide |
| Analyzing Compounds | Bromadiolone |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV-Vis Spectrum of Bromadiolone
July 22, 2024
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Bromadiolone check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Bromadiolone
July 11, 2017
Bromadiolone a rodenticide meant to kill rats and mice. It’s sold as an odorless powder with a light yellow tint of color. It is toxic if ingested orally since it the blood clotting due to the rodenticide not letting the body to reuse the K Vitamin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Bromadiolone. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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