HPLC Chiral Separation of Bromadiolone on Cyralis CM Column

HPLC Method for Analysis of Bromadiolone on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromadiolone.

Bromadiolone is a an anticoagulant with the chemical formula C30H23BrO4. It works as a rodenticide which has a tendency to accumulate in the liver of the poisoned organism. It is a second-generation 4-hydroxycoumarin derivative. Vitamin K1 is considered to be an antidote to the pesticide. You can find detailed UV spectra of Bromadiolone and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Bromadiolone enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.

Bromadiolone can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/TFA – 87/13/0.5%
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution(1-2): 9.90, (2-3): 1.69, (3-4): 2.20

Description

Class of CompoundsPesticide
Analyzing CompoundsBromadiolone

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bromadiolone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.