Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one

CAS Number2634-33-5
Molecular FormulaC7H5NOS
Molecular Weight151.19
LogP1.3
Synonyms
  • 1,2-Benzisothiazol-3(2H)-one
  • 2634-33-5
  • 1,2-Benzisothiazolin-3-one
  • 1,2-benzothiazol-3-one
  • benzisothiazolone
  • Benzo[d]isothiazol-3(2H)-one
  • Proxel
  • 1,2-Benzisothiazoline-3-one
  • Benzo[d]isothiazol-3-one
  • Proxel PL
  • Benzo[d]isothiazol-3-ol
  • 1,2-BENZISOTHIAZOL-3-ONE
  • Nipacide BIT
  • Proxel AB
  • 2,3-dihydro-1,2-benzothiazol-3-one
  • Proxel XL 2
  • benzoisothiazol-3-one
  • 1,2-benzoisothiazolin-3-one
  • 1,2-Benzisothiazolone
  • C7H5NOS
  • UNII-HRA0F1A4R3
  • 3-Hydroxy-1,2-benzisothiazole
  • IPX
  • CHEBI:167099
  • HRA0F1A4R3
  • 1,2-Benzoisothiazoline-3-one
  • 2,3-dihydro-3-oxo-1,2-benzisothiazole
  • MLS-0254244.0001
  • Benzo(d)isothiazol-3(2H)-one
  • Benzisothiazolin-3-one
  • Caswell No. 079A
  • Caswell No. 513A
  • Benzocil
  • 1,2-benzisothiazolinone
  • Acticide BIT
  • Canguard BIT
  • Denicide BIT
  • Proxel BD
  • Proxel CF
  • Proxel TN
  • Proxel XL
  • Proxel BDN
  • Proxel GXL
  • Proxel Ultra 5
  • San-aibac AP
  • Proxel LV-S
  • Proxel Press Paste
  • Apizas AP-DS
  • Acticide BW 20
  • Bestcide 200K
  • Nipacide BIT 20
  • Parmetol B 70
  • Parmetol D 11
  • Proxel GXL(S)
  • Proxel HL 2
  • Nuosept 485
  • Nuosept 491
  • Nuosept 495
  • Topcide 600
  • XBINX
  • CCRIS 6369
  • Denicide BIT 20N
  • Koralone B 119
  • Nipacide BIT 10W
  • Preventol BIT 20D
  • Proxel BD 20
  • Proxel Press Paste D
  • Troysan 1050
  • Bioban BIT 20DPG
  • benzisothiazoline-3-one
  • Canguard BIT 20DPG
  • EINECS 220-120-9
  • SD 202 (bactericide)
  • Canguard Ultra BIT 20LE
  • EPA Pesticide Chemical Code 098901
  • 1,2-Benzoisothiazol-3-one
  • BIT 10W
  • BIT 20
  • 2-Thiobenzimide
  • 2,3-Dihydrobenzisothiazol-3-one
  • 1,2-benzisothiazole-3(2h)-one
  • SD 202
  • 1,2-BENZISOTHAZOL-3(2H)-ONE
  • 1,2-Benzothiazolinone
  • Benzisothiazolone (BIT)
  • Epitope ID:115004
  • Benzisothiazol-3(2H)-one

Applications:

Uv-Vis Spectrum of Benzisothiazolinone

February 25, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Benzisothiazolinone (BIT) on Newcrom R1 Column

May 19, 2022

HPLC Method for Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one.

Benzisothiazolinone, also written as BIT, is a microbicide and fungicide with the chemical formula C7H5NOS. It is used primarily as a preservative in a large variety of industries, from paints, to caulks, to cleaning products, to agriculture, and more. It is banned from being used in cosmetics in the EU.  You can find detailed UV spectra of Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one and information about its various lambda maxima by visiting the following link.

Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a trifluoracetic acid buffer. Detection is performed using UV.

Condition
Column Newcrom R1, 3.2 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer TFA- 0.1%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.