HPLC Method for Analysis of Benzisothiazolinone (BIT) on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase.


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzisothiazolinone (BIT)

Acetic Anyhydride is a common reagent in organic synthesis, particularly in the acetylation of alcohols, amines, and aromatic rings. Benzyl Alcohol is a popular organic solvent and preservative used in topical drugs, cosmetics, and intravenous drugs. Benzoic Anhydride is the anhydride form of Benzoic Acid and a common reagent in organic synthesis. These 3 organic compounds can be separated and analyzed on a Newcrom R1 reverse-phase column using a simple gradient analytical method with a mobile phase consisting of just water and Acetonitrile (MeCN) (with no ionic modifier). This analysis method can be UV detected at 200 or 275 nm with high resolution and peak symmetry.

 

Condition
Column Newcrom R1, 3.2×50 mm,  100A
Mobile Phase MeCN – 10%
Buffer TFA- 0.1%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Benzisothiazolinone (BIT)

 

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Benzisothiazolinone (BIT), 1,2-benzisothiazol-3(2H)-one
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.