| CAS Number | 77-86-1 |
|---|---|
| Molecular Formula | C4H11NO3 |
| Molecular Weight | 121.136 |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| LogP | -2.9 |
| Synonyms |
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Applications:
HPLC Method for Separation of Trometamol (Tris) and Sodium on Primesep 100 Column
January 20, 2023
HPLC Method for Separation of Trometamol (Tris) and Sodium on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Trometamol, also known as Tris or Tromethamine, is a buffering agent (or Good’s buffer) frequently used in biochemical and molecular biological applicaitons. Tris is often mixed with Sodium Chloride as a buffer, and the Sodium ion and Tris will often co-elute, deliving inaccurate quantitation results. Tris and Sodium ion can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using several different isocratic analytical methods with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) buffer. By simply changing the relative concentrations of MeCN and Water, the retention times and order of Tris and Sodium can be significantly manipulated to deliver high quality separations. This analysis method is compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (LCMS).
High Performance Liquid Chromatography (HPLC) Method for Separation of Trometamol (Tris) and Sodium
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid – 1.0% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40C |
| Peak Retention Time |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Trometamol (Tris) |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
March 27, 2011
TRIS or tris(hydroxymethyl)aminomethane, is a hydrophilic basic compounds widely used in chemistry, biochemistry, and molecular biology. TRIS pKa (8.1) makes it an effective buffer in pH range of 7-9.2. TRIS is not retained on reversed-phase column and is not UV active, making it hard to be analyzed by HPLC. TRIS was analyzed by mixed-mode HPLC on a Primesep 100 column. Retention time of compound is controlled by the amount and pH of the buffer. No ion-pairing reagent is required for HPLC retention of hydrophilic basic molecules like TRIS. TRIS can be monitored by ELSD/CAD with trifluoroacetic acid in the mobile phase, or by LC/MS with ammonium formate or acetate in the mobile phase.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
| Column | Primesep D, Primesep 100 |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid |
| Flow Rate | 0.5 , 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Amine |
| Analyzing Compounds | Tris (Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM) |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTrometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
