HPLC Method for Separation of Trometamol (Tris) and Sodium on Primesep 100 Column

HPLC Method for Separation of  Trometamol (Tris) and Sodium on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of  Trometamol (Tris) and Sodium on Primesep 100 Column by SIELC Technologies
HPLC Method for Separation of  Trometamol (Tris) and Sodium on Primesep 100 Column by SIELC Technologies

Trometamol, also known as Tris or Tromethamine, is a buffering agent (or Good’s buffer) frequently used in biochemical and molecular biological applicaitons. Tris is often mixed with Sodium Chloride as a buffer, and the Sodium ion and Tris will often co-elute, deliving inaccurate quantitation results. Tris and Sodium ion can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using several different isocratic analytical methods with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) buffer. By simply changing the relative concentrations of MeCN and Water, the retention times and order of Tris and Sodium can be significantly manipulated to deliver high quality separations. This analysis method is compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (LCMS).

High Performance Liquid Chromatography (HPLC) Method for Separation of  Trometamol (Tris) and Sodium

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O
BufferFormic Acid – 1.0%
Flow Rate1.0 ml/min
DetectionELSD, 40C
Peak Retention Time

Description

Class of CompoundsDrug
Analyzing CompoundsTrometamol (Tris)
Application Analytes:
Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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