HPLC Method for Separation of  Trometamol (Tris) and Sodium on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Trometamol, also known as Tris or Tromethamine, is a buffering agent (or Good’s buffer) with the chemical formula C₄H₁₁NO₃. It is frequently used in biochemical and molecular biological applications as a component of buffer solutions. Tris is often mixed with Sodium Chloride as a buffer, and the Sodium ion and Tris will often co-elute, delivering inaccurate quantitation results. Due to its ability to increase permeability of cell membranes, it is a component of the Moderna COVID-19 vaccine as well as the Pfizer-BioNTech COVID-19 children’s vaccine.

Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM) can be retained, separated, and analyzed on a Primesep 100 mixed-mode stationary phase column using several different isocratic analytical methods with a simple mobile phase of water, Acetonitrile (MeCN), and a Formic acid (FA) buffer. By simply changing the relative concentrations of MeCN and Water, the retention times and order of Tris and Sodium can be significantly manipulated to deliver high quality separations. This analysis method is compatible with evaporative detection methods such as Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (LCMS).

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