Trisamine

Trisamine structural formula

CAS Number77-86-1
Molecular FormulaC4H11NO3
Molecular Weight121.137
InChI KeyLENZDBCJOHFCAS-UHFFFAOYSA-N
LogP-1.80
Synonyms
  • Tromethamine
  • 2-Amino-2-(hydroxymethyl)propane-1,3-diol
  • 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-
  • 77-86-1
  • 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-
  • Tris(hydroxymethyl)aminomethane
  • [2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]amine
  • 2-Amino-1,3-dihydroxy-2-(hydroxymethyl)propane
  • 2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANDIOL
  • 2-Amino-2-(hydroxymethyl)-1,3-propanediol
  • 2-Amino-2-(hydroxymethyl)propane-1,3-diol
  • 2-Amino-2-hydroxymethyl-1, 3-propanediol
  • 2-amino-2-hydroxymethyl-1,3-propanediol
  • 2-Amino-2-hydroxymethylpropan-1,3-diol
  • 2-Amino-2-methylol-1,3-propanediol
  • Addex-Tham
  • Aminotri(hydroxymethyl)methane
  • Aminotrimethylolmethane
  • Aminotris(hydroxymethyl)methane
  • Methanamine, 1,1,1-tris(hydroxymethyl)-
  • NSC 103026
  • NSC 6365
  • NSC 65434
  • Pehanorm
  • PROPANE-1,3-DIOL, 2-AMINO-2-(HYDROXYMETHYL)-
  • Talatrol
  • Tri Amino
  • Tri(hydroxymethyl)methylamine
  • Trigmo base
  • TRIHYDROXYMETHYLAMINOMETHANE
  • Triladyl
  • Trimethylolaminomethane
  • TRIS
  • Tris Amino
  • Tris Amino Crystal
  • Tris base
  • TRIS BUFFER
  • Tris(hydroxymethyl)methanamine
  • TRIS(HYDROXYMETHYL)METHYLAMINE
  • Tris(methylolamino)methane
  • Tris, free base
  • Trisamin
  • Trisamine
  • Trisaminol
  • Trishydroxymethylaminomethane
  • Trispuffer
  • Tris-steril
  • Trometamol
  • Trometamole
  • Tromethane
  • Tromethanmin
  • Tutofusin tris
  • Apiroserum Tham
  • Caswell No. 036
  • EINECS 201-064-4
  • EPA Pesticide Chemical Code 083901
  • 2-(Hydroxymethyl)-2-amino-1,3-propanediol
  • Methylamine, 1,1,1-tris(hydroxymethyl)-
  • Tris-hydroxymethylaminomethane
  • Tromethamolum
  • Tris-hydroxymethyl-aminomethan
  • Trometamolum
  • UNII-023C2WHX2V
  • 1,1,1-tris(hydroxymethyl)methanamine
  • THAM
  • Tris-base
  • tris(hydroxymethyl)aminomethane
  • 108195-86-4
  • 1158650-64-6
  • 119320-15-9
  • 25149-07-9
  • 68755-45-3
  • 83147-39-1
  • 857365-23-2

Applications:

HPLC Method for Analysis of Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)

March 27, 2011


TRIS or tris(hydroxymethyl)aminomethane, is a hydrophilic basic compounds widely used in chemistry, biochemistry, and molecular biology. TRIS pKa (8.1) makes it an effective buffer in pH range of 7-9.2. TRIS is not retained on reversed-phase column and is not UV active, making it hard to be analyzed by HPLC. TRIS was analyzed by mixed-mode HPLC on a Primesep 100 column. Retention time of compound is controlled by the amount and pH of the buffer. No ion-pairing reagent is required for HPLC retention of hydrophilic basic molecules like TRIS. TRIS can be monitored by ELSD/CAD with trifluoroacetic acid in the mobile phase, or by LC/MS with ammonium formate or acetate in the mobile phase.



 

High Performance Liquid Chromatography (HPLC) Method for Analyses of Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)




Condition
Column Primesep D, Primesep 100
Mobile Phase MeCN/H2O
Buffer  Formic Acid
Flow Rate 0.5 , 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Amine
Analyzing Compounds Tris (Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Trisamine
Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.