HPLC Method for Analysis of Atrolactic Acid on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Atrolactic Acid.

Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C₉H₁₀O₃. It can cause irritation to eyes, skin, and the respiratory system. You can find detailed UV spectra of Atrolactic Acid and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Atrolactic Acid enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Atrolactic Acid can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

Description

If you are looking for optimized HPLC method to analyze Atrolactic Acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Atropine, Atrolactic Acid, Pralidoxime Chloride on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Atropine, Atrolactic Acid, Pralidoxime Chloride.

Atropine is a tropane alkaloid with the chemical formula C17H23NO3. It is an anticholinergic medication that is used to treat nerve agent, pesticide poisoning, slow heartrate, and is also used to decrease saliva production. Typically, it is administered through injection.

Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C₉H₁₀O₃. It can cause irritation to eyes, skin, and the respiratory system.

Pralidoxime Chloride, also known as 2-PAM, is a cholinesterase reactivator with the chemical formula C₇H₉N₂O⁺. It is used as an antidote for poisoning by organophosphate pesticides and nerve agents.

Atropine, Atrolactic Acid, Pralidoxime Chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

HPLC Method for Atrolactic Acid on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Atrolactic Acid.

Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C₉H₁₀O₃. It can cause irritation to eyes, skin, and the respiratory system.

Atrolactic Acid can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.