HPLC Method for Atrolactic Acid on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Atrolactic Acid.
Atrolactic Acid is a 2-hydroxy monocarboxylic acid with the chemical formula C9H10O3. It can cause irritation to eyes, skin, and the respiratory system.
Atrolactic Acid can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Acid, Hydrophobic |
| Analyzing Compounds | Atrolactic Acid |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Atrolactic AcidApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
