| CAS Number | 59277-89-3 |
|---|---|
| Molecular Formula | C8H11N5O3 |
| Molecular Weight | 225.208 |
| InChI Key | MKUXAQIIEYXACX-UHFFFAOYSA-N |
| LogP | -1.56 |
| Synonyms |
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Applications:
HPLC Method for Simultaneous Determination of Acyclovir and Hydrocortisone on Primesep 100 Column
August 31, 2022
HPLC Method for Acyclovir, Hydrocortisone on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Hydrocortisone.
Acyclovir, also known as Aciclovir, is an anti-viral medication with the chemical formula C8H11N5O3. It is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. It can also be used to treat chickenpox in children. After it was discovered, Acyclovir was considered the start of a new era in antiviral medicine.
Hydrocortisone is a common corticosteroid with the molecular formula C21H30O5. A corticosteroid medication used to treat various conditions by reducing inflammation and suppressing the immune system. It is used for conditions such as adrenogenital syndrome, thyroiditis, dermatitis, asthma, and more. It is on the World Health Organization’s List of Essential Medicines. Hydrocortisone is effective in reducing mortality rate of critically ill COVID-19 patients. You can find detailed UV spectra of Hydrocortisone and information about its various lambda maxima by visiting the following link.
Acyclovir, Hydrocortisone can be separated, retained, and analyzed on a Primesep 100 column mixed-mode column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 254 nm with high resolution and peak symmetry.
| Column | Primesep 100, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 35% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 254 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Acyclovir, Hydrocortisone |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Hydrocortisone
HPLC Separation of Antiviral Drugs on Primesep 100 Column
April 1, 2021
HPLC Method for Acyclovir, Valacyclovir, Oseltamivir on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Valacyclovir, Oseltamivir.
Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Gradient H2SO4 – 0.05- 0.8%, 10 min |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Acyclovir, Valacyclovir, Oseltamivir |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Oseltamivir
Valacyclovir
Vis Detection
HPLC Analysis of Acyclovir
August 6, 2015
Acyclovir (also spelled aciclover) is an anti-viral drug on the World Health Organization list of essential medicines due to its use in the prevention and treatment of herpes simplex virus, varicella zoster virus and the shingles. Legacy L1 was used to retain aciclovir by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
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