HPLC Method for Simultaneous Determination of Acyclovir and Hydrocortisone on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir and Hydrocortisone

Acyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. It can also be used to treat chickenpox in children. Hydrocortisone, or cortisol, is a common corticosteroid used to treat a wide variety of ailments, including high blood calcium, arthritis, dermatitis, and asthma, among others. Both of these drugs can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 254 nm with high resolution and peak symmetry.

Condition
Column Primesep 100, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN – 35%
Buffer H2SO4 – 0.1%
Flow Rate 0.5 ml/min
Detection UV 254 nm

 

Description
Class of Compounds Drug
Analyzing Compounds Acyclovir and Hydrocortisone

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acyclovir
Hydrocortisone
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.