Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Tofacitinib check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for Tofacitinib on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofacitinib

Tofacitinb, known under its brand name Xeljanz, is a medication  with the medical formula C₁₆H₂₀N₆O. It is commonly used to treat rheumatoid arthritis, psoriatic arthritis, and ulcerative colitis. Tofacitinib can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.

Tofacitinib can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.