| CAS Number | 477600-75-2 |
|---|---|
| Molecular Formula | C16H20N6O |
| Molecular Weight | 312.37 |
| InChI Key | UJLAWZDWDVHWOW-YPMHNXCESA-N |
| Synonyms |
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Applications:
HPLC Method for Determination of Tofacitinib on Newcrom R1 Column
September 12, 2022
Separation type: Liquid Chromatography Reverse Phase
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofacitinib
Tofacitinb, known under its brand name Xeljanz, is a medication commonly used to treat rheumatoid arthritis, psoriatic arthritis, and ulcerative colitis. Tofacitinib can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.| Column | Newcrom R1 2.1×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN – 15% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 290 nm |
| LOD | 0.02 ppm |
| Class of Compounds | Drug |
| Analyzing Compounds | Tofacitinib |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsApplication Analytes:
Tofacitinib
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
