Tofacitinib

CAS Number477600-75-2
Molecular Formula C16H20N6O
Molecular Weight312.37
InChI KeyUJLAWZDWDVHWOW-YPMHNXCESA-N
Synonyms
  • CP 690,550
  • CP 690550
  • CP-690,550
  • CP-690550
  • CP690550
  • tasocitinib
  • tofacitinib
  • tofacitinib citrate
  • Xeljanz

Applications:

HPLC Method for Determination of Tofacitinib on Newcrom R1 Column

September 12, 2022

HPLC Method for Tofacitinib on Newcrom R1 by SIELC Technologies

     

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofacitinib

Tofacitinb, known under its brand name Xeljanz, is a medication  with the medical formula C16H20N6O. It is commonly used to treat rheumatoid arthritis, psoriatic arthritis, and ulcerative colitis. Tofacitinib can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.

Tofacitinib can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN – 15%
Buffer H2SO4 – 0.2%
Flow Rate 0.2 ml/min
Detection UV 290 nm
LOD 0.02 ppm

 

Description
Class of Compounds Drug
Analyzing Compounds Tofacitinib

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tofacitinib
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.