HPLC Method for Determination of Tofacitinib on Newcrom R1 Column

Separation type: Liquid Chromatography Reverse Phase

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofacitinib

Tofacitinb, known under its brand name Xeljanz, is a medication commonly used to treat rheumatoid arthritis, psoriatic arthritis, and ulcerative colitis. Tofacitinib can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 290 nm with high resolution and peak symmetry.

Condition
Column Newcrom R1 2.1×100 mm, 3 µm, 100A
Mobile Phase MeCN – 15%
Buffer H2SO4 – 0.2%
Flow Rate 0.2 ml/min
Detection UV 290 nm
LOD 0.02 ppm

 

Description
Class of Compounds Drug
Analyzing Compounds Tofacitinib

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Tofacitinib
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.