3-Methylpyrazole

3-Methylpyrazole structural formula

CAS Number1453-58-3
Molecular FormulaC4H6N2
Molecular Weight82.107
InChI KeyXKVUYEYANWFIJX-UHFFFAOYSA-N
LogP0.509
Synonyms
  • 3-Methylpyrazole
  • 3-Methyl-1H-pyrazole
  • 1H-Pyrazole, 3-methyl-
  • 1453-58-3
  • 5-23-05-00004
  • BRN 0001454
  • EINECS 215-925-7
  • 3(5)-Methylpyrazole
  • 5-Methylpyrazole
  • Pyrazole, 5-methyl-
  • UNII-W7KU1RRO6U

Applications:

HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column

December 20, 2019

HPLC Method for Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 by SIELC Technologies

HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column Column_1211

High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole 

Dicyandiamide, or cyanoguanidine, is used as a curing agent for epoxy resins. 1,2,4-triazole, is a heterocycle used primarily as an antifungal but has other uses in the pharmaceutical industry as well. 3-methylpyrazole is used in nitrogen fertilizers. All three compounds are structurally similar and can be separated in HPLC using Primesep 100 reverse-phase (RP) mixed-mode cation-exchange (CX) column using acetonitrile (ACN) and water mobile phase with phosphoric acid buffer and UV detected at 200nm and 220nm.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200, 220 nm

Description
Class of Compounds Heterocycle, Amine, Hydrophilic, Ionizable
Analyzing Compounds Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
1,2,4-Triazole
3-Methylpyrazole
Dicyandiamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of 3-Methylpyrazole on Newcrom R1 HPLC column

February 16, 2018
Separation of 3-Methylpyrazole on Newcrom C18 HPLC column

3-Methylpyrazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
3-Methylpyrazole
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.