| CAS Number | 1453-58-3 |
|---|---|
| Molecular Formula | C4H6N2 |
| Molecular Weight | 82.107 |
| InChI Key | XKVUYEYANWFIJX-UHFFFAOYSA-N |
| LogP | 0.509 |
| Synonyms |
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Applications:
HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column
December 20, 2019
Dicyandiamide, or cyanoguanidine, is used as a curing agent for epoxy resins. 1,2,4-triazole, is a heterocycle used primarily as an antifungal but has other uses in the pharmaceutical industry as well. 3-methylpyrazole is used in nitrogen fertilizers. All three compounds are structurally similar and can be separated in HPLC using Primesep 100 reverse-phase (RP) mixed-mode cation-exchange (CX) column using acetonitrile (ACN) and water mobile phase with phosphoric acid buffer and UV detected at 200nm and 220nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 220 nm |
| Class of Compounds | Heterocycle, Amine, Hydrophilic, Ionizable |
| Analyzing Compounds | Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options3-Methylpyrazole
Dicyandiamide
Separation of 3-Methylpyrazole on Newcrom R1 HPLC column
February 16, 2018
3-Methylpyrazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options