1,2,4-Triazole

Molecular Formula C2H3N3
Molecular Weight69.07
InChI KeyNSPMIYGKQJPBQR-UHFFFAOYSA-N
LogP-0.6
Synonyms
  • 1H-1,2,4-Triazole
  • 1,2,4-TRIAZOLE
  • 288-88-0
  • 4H-1,2,4-triazole
  • Pyrrodiazole
  • s-Triazole
  • 1,2,4-1H-Triazole
  • 63598-71-0
  • UNII-10MS0Y1RDI
  • MFCD00005228
  • NSC 83128
  • 1,2,4 triazole
  • 1,2,4-triazol
  • 4H-1,2,4-Triazole (VAN)
  • Peptone, bacteriological
  • EINECS 206-022-9
  • 1H-[1,2,4]triazole
  • 10MS0Y1RDI
  • AI3-51031
  • DTXSID6027131
  • CHEBI:46077
  • NSPMIYGKQJPBQR-UHFFFAOYSA-N
  • (1,2,4)-triazole
  • [1,2,4]-triazole
  • 1H-1,2,4-triazol
  • 1,2,4-1H-Triazole, 99.5%
  • InChI=1/C2H3N3/c1-3-2-5-4-1/h1-2H,(H,3,4,5
  • HSDB 7860
  • 1,4-Triazole
  • 1,2,4triazole
  • 1,3,4-triazole
  • 1H-1,4-Triazole
  • 4H-1,4-Triazole
  • [1,2,4]triazole
  • 1,2, 4-triazole
  • 1,2,4,-triazole
  • PubChem17626
  • [1,2,4]triazol
  • ACMC-1BLVK
  • [1.2.4]-triazole
  • 1, 2, 4 triazole
  • [1,2,4]-triazol
  • 1,2,4-1h triazole
  • DSSTox_CID_7131
  • EC 206-022-9
  • 4H-[1,2,4]Triazole
  • DSSTox_RID_78318
  • DSSTox_GSID_27131
  • 1H-[1,2,4]-triazole
  • 1H-1, 2, 4 Triazole
  • KSC201Q7N
  • CHEMBL15571
  • 1,2,4-Triazole, 98%
  • NIOSH/XZ3807000
  • CHEBI:35550
  • CTK1A1876
  • BCP20885
  • CS-D1150
  • KS-00000E5P
  • NSC83128
  • ZINC5943507
  • Tox21_300113
  • ANW-26493
  • CGA-71019
  • MFCD01941334
  • NSC-83128
  • SBB040922
  • STK366100
  • 1,2,4-Triazole, analytical standard
  • AKOS000120326
  • AKOS000269054
  • DB03594
  • MCULE-3522189420
  • MP-2132
  • PS-9377
  • RTR-012666
  • TRA0010871
  • NCGC00247903-01
  • NCGC00254087-01
  • 736917-78-5
  • AK111541
  • BP-12667
  • CAS-288-88-0
  • SC-09249
  • SY001414
  • AB1003725
  • DB-002017
  • LS-155746
  • LS-155747
  • ST2408887
  • TR-012666
  • FT-0607865
  • ST50214528
  • XZ38070000
  • 88T880
  • T-6200
  • 85482-EP2269993A1
  • 85482-EP2305657A2
  • 85482-EP2374791A1
  • 95526-EP2274983A1
  • 95526-EP2305672A1
  • MFCD00005228 (97+%)
  • 1,2,4-Triazole, Vetec(TM) reagent grade, 98%
  • A819652
  • Q161300
  • F1918-0085
  • Z1245636363

Applications:

HPLC Method for Separation of 1,2,4-Triazole and Guanylurea on Primesep 100 Column

January 9, 2024

HPLC Method for Analysis of Guanylurea, 1,2,4-Triazole on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Guanylurea, 1,2,4-Triazole on Primesep 100 Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Guanylurea, 1,2,4-Triazole

Guanylurea, also known as guanidylurea, is a chemical compound with the molecular formula (C_3H_7N_5O). It is derived from guanidine and urea, and it has interesting applications in the field of chemistry and materials science.

  • Crystal Growth: Guanylurea is known for its use in crystal growth processes. It has been utilized as an additive in solution to control and promote the growth of certain crystals, including organic and inorganic crystals. Guanylurea is part of the broader field of crystal engineering, where researchers explore the design and synthesis of crystalline materials with specific properties. The ability of guanylurea to form hydrogen bonds makes it relevant in this context.
  • Hydrogen Bonding Studies: Due to its unique structure, guanylurea has been studied for its hydrogen bonding properties, which are important in various chemical and biological processes.The presence of amino groups in guanylurea allows it to participate in hydrogen bonding interactions, which are crucial in determining the structure and properties of molecular assemblies.

It’s important to note that guanylurea’s applications are primarily in the realm of crystal engineering and related studies. Its ability to participate in hydrogen bonding interactions makes it a valuable component in the design and manipulation of crystalline materials for various purposes.

1,2,4-triazole is a heterocyclic compound with a unique five-membered ring structure. Its versatility, biological activities, and ability to coordinate with metal ions make it a compound of interest in various scientific and medicinal applications.

The 1,2,4-triazole and Guanylurea be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 200 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 10/90%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
SamplesGuanylurea – 0.77mg/mL
1,2,4-Triazole – 3.9mg/mL
Injection volume3 µl
LOD*Guanylurea – 5 ppb
1,2,4-Triazole – 25 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Aromatic amines, Ureas
Analyzing CompoundsGuanylurea, 1,2,4-Triazole

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1,2,4-Triazole
Guanylurea

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column

December 20, 2019


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HPLC Separation of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 Column Column_1211

Dicyandiamide, or cyanoguanidine, is used as a curing agent for epoxy resins. 1,2,4-triazole, is a heterocycle used primarily as an antifungal but has other uses in the pharmaceutical industry as well. 3-methylpyrazole is used in nitrogen fertilizers. All three compounds are structurally similar and can be separated in HPLC using Primesep 100 reverse-phase (RP) mixed-mode cation-exchange (CX) column using acetonitrile (ACN) and water mobile phase with phosphoric acid buffer and UV detected at 200nm and 220nm.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 10%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200, 220 nm

Description

Class of Compounds Heterocycle, Amine, Hydrophilic, Ionizable
Analyzing Compounds Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
1,2,4-Triazole
3-Methylpyrazole
Dicyandiamide
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC of Determination of 1,2,4-Triazole on Primesep 100 Column

December 17, 2019


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HPLC of Determination 1,2,4-Triazole on Primesep 100 Column_1209

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200 nm

 

Description

Class of Compounds
Heterocyclic, Hydrophilic, Base
Analyzing Compounds   1,2,3-Triazole

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
1,2,4-Triazole
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Triazole acetic acid and 1,2,4-Triazole on Primesep 100 Column

December 13, 2019


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Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer TFA – 0.1%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

 

Description

Class of Compounds
Heterocyclic, Hydrophilic, Base
Analyzing Compounds Triazole acetic acid,  1,2,3-Triazole

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
1,2,4-Triazole
Triazole acetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.