HPLC Method for Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole
Dicyandiamide, or cyanoguanidine, is used as a curing agent for epoxy resins. 1,2,4-triazole, is a heterocycle used primarily as an antifungal but has other uses in the pharmaceutical industry as well. 3-methylpyrazole is used in nitrogen fertilizers. All three compounds are structurally similar and can be separated in HPLC using Primesep 100 reverse-phase (RP) mixed-mode cation-exchange (CX) column using acetonitrile (ACN) and water mobile phase with phosphoric acid buffer and UV detected at 200nm and 220nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 220 nm |
| Class of Compounds | Heterocycle, Amine, Hydrophilic, Ionizable |
| Analyzing Compounds | Dicyandiamide, 1,2,4-Triazole, 3-Methylpyrazole |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
1,2,4-Triazole3-Methylpyrazole
Dicyandiamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
