| CAS Number | 37971-36-1 |
|---|---|
| Molecular Formula | C7H11O9P |
| Molecular Weight | 270.131 |
| InChI Key | SZHQPBJEOCHCKM-UHFFFAOYSA-N |
| LogP | -1.59 |
| Synonyms |
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Applications:
HPLC Determination of 2-Phosphonobutane-1,2,4-tricarboxylic on Newcrom BH Column
January 18, 2021
HPLC Method for 2-Phosphonobutane-1,2,4-tricarboxylic on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Phosphonobutane-1,2,4-tricarboxylic.
PBTC (2-phosphonobutane-1,2,4-tricarboxylic acid) is one of the most widely used scale inhibitors in the cooling water treatment industry. It works through binding with metal ions, therefore preventing them from forming scale. It is widely used especially due to performing well under harsh conditions like high temperatures and high alkalinity.
2-Phosphonobutane-1,2,4-tricarboxylic can be detected at low UV. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with phosphoric acid (H3PO4) buffer, 2-phosphonobutane-1,2,4-tricarboxylic acid can be retained and UV detected at 220nm.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H3PO4 – 1.0% |
| Flow Rate | 1 ml/min |
| Detection | UV 220 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | 2-Phosphonobutane-1,2,4-tricarboxylic |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of 2-Phosphono-1,2,4-butanetricarboxylic acid on Newcrom R1 HPLC column
February 16, 2018
2-Phosphono-1,2,4-butanetricarboxylic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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