HPLC Determination of 2-Phosphonobutane-1,2,4-tricarboxylic on Newcrom BH Column

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PBTC (2-phosphonobutane-1,2,4-tricarboxylic acid) is one of the most widely used scale inhibitors in the cooling water treatment industry.  PBTC can be detected at low UV.  Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with phosphoric acid (H3PO4) buffer, 2-phosphonobutane-1,2,4-tricarboxylic acid can be retained and UV detected at 220nm.

PBTC (2-phosphonobutane-1,2,4-tricarboxylic acid), one of the most widely used scale inhibitors in the cooling water treatment industry.


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4 – 1.0%
Flow Rate 1 ml/min
Detection UV 220 nm



Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds 2-Phosphonobutane-1,2,4-tricarboxylic


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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