| CAS Number | 87-28-5 |
|---|---|
| Molecular Formula | C9H10O4 |
| Molecular Weight | 182.176 |
| InChI Key | LVYLCBNXHHHPSB-UHFFFAOYSA-N |
| LogP | 1.39 |
| Synonyms |
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Applications:
UV-Vis Spectrum of 2-hydroxyethyl Salicylate
August 1, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column
August 14, 2019
HPLC Method for 2-Hydroxyethyl salicylate, Salicylic acid on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Hydroxyethyl salicylate, Salicylic acid
2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.
2-Hydroxyethyl salicylate, Salicylic acid can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep B |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 238 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol |
| Analyzing Compounds | 2-Hydroxyethyl salicylate, Salicylic acid |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Salicylic acid
Separation of 2-Hydroxyethyl salicylate on Newcrom R1 HPLC column
February 16, 2018
2-Hydroxyethyl salicylate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
