| CAS Number | 451-82-1 |
|---|---|
| Molecular Formula | C8H7FO2 |
| Molecular Weight | 154.14 |
| InChI Key | RPTRFSADOICSSK-UHFFFAOYSA-N |
| LogP | 1.5 |
| Synonyms |
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Applications:
HPLC Method for 2-Fluorophenylacetic acid, 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid Compatible with Mass Spectrometry
October 1, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Fluorophenylacetic acid , 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid
2-Fluorophenylacetic acid is a popular chiral derivatizing agent used in 19F NMR spectroscopy. 3-Fluorophenylacetic acid is a common precursor for synthesizing various pentaamine and bis-heterocyclic compounds. 4-Fluorophenylacetic acid is a common intermediary compound used to produce fluorinated anesthetics. While these 3 compounds all have the same chemical formula, the position of the Fluoride group varies between them, changing slightly their retention and chromatographic characteristics.
All 3 isomers can be measured at low UV. Using a Primsep SB reverse-phase column and a mobile phase consisting of acetonitrile (MeCN) and water with either formic acid or acetic acid buffer, these fluorophenylacetic acid isomers can be retained, separated, and UV detected at 264 nm with precise resolution. This method is compatible with Mass Spectrometry.
| Column | Primesep SB, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 264 nm |
| Column | Primesep SB, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | Acetic Acid – 2.0% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 264 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 2-Fluorophenylacetic acid (2-FPAA), 4-Fluorophenylacetic acid (4-FPAA), 3-Fluorophenylacetic acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options3-Fluorophenylacetic acid
4-Fluorophenylacetic acid
HPLC Separation of 2-Fluorophenylacetic acid, 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid on Primesep SB Column
September 29, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Fluorophenylacetic acid , 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid
2-Fluorophenylacetic acid is a popular chiral derivatizing agent used in 19F NMR spectroscopy. 3-Fluorophenylacetic acid is a common precursor for synthesizing various pentaamine and bis-heterocyclic compounds. 4-Fluorophenylacetic acid is a common intermediary compound used to produce fluorinated anesthetics. While these 3 compounds all have the same chemical formula, the position of the Fluoride group varies between them, changing slightly their retention and chromatographic characteristics.
All 3 isomers can be measured at low UV. Using a Primsep SB reverse-phase columns and a mobile phase consisting of acetonitrile (MeCN) and water with either formic acid or acetic acid buffer, these fluorophenylacetic acid isomers can be retained, separated, and UV detected at 210nm with precise resolution.
| Column | Newcrom BH, 4.6×250 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Column | Primesep SB, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 2-Fluorophenylacetic acid (2-FPAA), 4-Fluorophenylacetic acid (4-FPAA), 3-Fluorophenylacetic acid |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
3-Fluorophenylacetic acid
4-Fluorophenylacetic acid
HPLC Separation of 2-Fluorophenylacetic acid, 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid on Newcrom BH Column
June 5, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Fluorophenylacetic acid , 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid
2-Fluorophenylacetic acid is a popular chiral derivatizing agent used in 19F NMR spectroscopy. 3-Fluorophenylacetic acid is a common precursor for synthesizing various pentaamine and bis-heterocyclic compounds. 4-Fluorophenylacetic acid is a common intermediary compound used to produce fluorinated anesthetics. While these 3 compounds all have the same chemical formula, the position of the Fluoride group varies between them, changing slightly their retention and chromatographic characteristics.
All 3 isomers can be measured at low UV. Using a Newcrom BH reverse-phase columns and a mobile phase consisting of acetonitrile (MeCN) and water with either formic acid or acetic acid buffer, these fluorophenylacetic acid isomers can be retained, separated, and UV detected at 210 nm with precise resolution.
| Column | Newcrom BH, 4.6×250 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H3PO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 2-Fluorophenylacetic acid (2-FPAA), 4-Fluorophenylacetic acid (4-FPAA), 3-Fluorophenylacetic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 3 µm
Pore Size: 100 A
3-Fluorophenylacetic acid
4-Fluorophenylacetic acid
