HPLC Separation of 2-Fluorophenylacetic acid, 4-Fluorophenylacetic acid, 3-Fluorophenylacetic acid on Newcrom BH and Primesep SB Columns

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud   High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Fluorophenylacetic acid , 4-Fluorophenylacetic acid,  3-Fluorophenylacetic acid 2-Fluorophenylacetic acid is a popular chiral derivatizing agent used in 19F NMR spectroscopy. 3-Fluorophenylacetic acid is a common precursor for synthesizing various pentaamine and bis-heterocyclic compounds. 4-Fluorophenylacetic acid is a common intermediary compound used to produce fluorinated anesthetics. While these 3 compounds all have the same chemical formula, the position of the Fluoride group varies between them, changing slightly their retention and chromatographic characteristics. All 3 isomers can be measured at low UV. Using a Primsep SB and a Newcrom BH reverse-phase columns and a mobile phase consisting of acetonitrile (MeCN) and water with either formic acid or acetic acid buffer, these fluorophenylacetic acid isomers can be retained, separated, and UV detected at 264 nm with precise resolution. This method is compatible with Mass Spectrometry.  

Condition 1

Column Newcrom BH, 4.6x250 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer H3PO4 - 0.05%
Flow Rate 1.0 ml/min
Detection UV 210  nm

Condition 2

Column Primesep SB, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV 210  nm


Class of Compounds Acid
Analyzing Compounds 2-Fluorophenylacetic acid (2-FPAA), 4-Fluorophenylacetic acid (4-FPAA), 3-Fluorophenylacetic acid
Application Analytes:
2-Fluorophenylacetic acid
3-Fluorophenylacetic acid
4-Fluorophenylacetic acid