| CAS Number | 7305-71-7 |
|---|---|
| Molecular Formula | C4H6N2S |
| Molecular Weight | 114.170 |
| InChI Key | GUABFMPMKJGSBQ-UHFFFAOYSA-N |
| LogP | 0.426 |
| Synonyms |
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Applications:
Separation of Model Compounds in Reversed-Phase and Mixed-Mode
April 25, 2019
HPLC Method for Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole.
Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.
| Column | Solid-Core Primesep 100, 3.2 x 50 mm, 2.7 µm, 90 A, dual ended |
| Mobile Phase | Gradient MeCN – 10-60%, 5 min |
| Buffer | Gradient AmFm pH 3.5- 30 – 70 mM, 5 min |
| Flow Rate | 1.2 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Basic, Hydrophilic, Hydrophobic, Ionizable. |
| Analyzing Compounds | Adenosine, 3,4-Difluoroaniline, 4-Amino-2-Chloropyridine, 4-Amino-3-Chloropyridine, 2-Amino-5-Methylthiadiazole, 2-Amino-5-methyl-thiazole, 4-Ethylaniline, 5-Aminoindole |
Application Column
Solid-Core Primesep 100
Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 2.7 µm
Pore Size: 90 A
Attribute: none
Column options: dual ended
2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine
Separation of 2-Amino-5-methyl-thiazole on Newcrom R1 HPLC column
May 16, 2018
2-Amino-5-methyl-thiazole can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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